New Method for Analyzing Tyramine by Spectrofluorimetry: Application to Fish

A new method for direct spectrofluorimetric analysis of tyramine in various fish samples was established. Low limits of detection (LOD) between 0.53 ng/mL and 1.40 ng/mL and limits of quantification (LOQ) between 1.76 ng/mL and 4.68 ng/mL were obtained. Also, low values of relative standard deviations (RSD) between 0.30 % and 0.58 % were obtained; confirming good reproducibility of our measurements. The application of this method to our samples allowed the detection of tyramine with satisfactory recovery rates between 93.68 % and 106.60 %. Interference studies with different biogenic amines have shown that the determination of tyramine can be done without major problems in the presence of spermine, spermidine, and histamine. However, special attention should be paid when tyramine is determined in the presence of agmatine, cadaverine or putrescine. In all cases, the determination of tyramine is more influenced by the presence of tryptamine or dopamine in the sample.