Antioxidant and toxicological activities of Pyrossia lanceolata (L.) Farw. extracts

Background: Pyrrosia lanceolata (L.) farw. is one of the Polypodiaceae species that is commonly used as a traditional medicinal plant. Objective: The purpose of this study was to determine the antioxidant and toxic levels in the extracts of the species. Method: The aerial portion of the specie was extracted with methanol, followed by a liquid-liquid extraction with n-hexane, dichloromethane, and ethyl acetate. DPPH radicals and the brine shrimp lethality test, , were used to assess its antioxidant activity and toxicity. Result: The n-hexane extract exhibited no activity with IC50 values exceeding 500 μg/mL, whereas the ethyl acetate extract exhibited a high level of antioxidant activity with an IC50 value of 12.08±0.27 μg/mL. Moreover, when tested against Artemia salina leach, the toxicological level of the extracts was greater than 1000 μg/mL. Conclusion: These findings lay the groundwork for further investigation into the process of isolating and evaluating the biological activity of secondary metabolites that were discovered in the extract.