2022•PubMedOpen access

[Hydroxynitone suppresses hepatic stellate cell activation by inhibiting TGF-β1 phosphorylation to alleviate CCl4-induced liver fibrosis in rats].

Zhenzhen Zhao, Hanh Nguyen Dong, Bohu Li, Bingbing Shen, Yang Guo, Tengfei Gu, Ying Qu, Xiu-jun Cai, Ling Lü

Abstract

OBJECTIVE: -induced liver fibrosis in rats and explore the possible mechanism. METHODS: in all but the control group. Hydronidone and pirfenidone were given daily at the indicated doses by intragastric administration for 6 weeks. After the treatments, serum samples were collected from the rats for detecting liver function parameters, and hydroxyproline content in the liver tissue was determined. Inflammation and fibrosis in the liver tissue were observed using HE staining and Sirius Red staining. In the cell experiment, human hepatic stellate cell line LX-2 was stimulated with TGF-β1 and treated with hydronidone or pirfenidone, and the expression levels of α-SMA, collagen type I and phosphorylated Smad3, phosphorylated p38, phosphorylated ERK1/2 and phosphorylated Akt were detected with Western blotting. RESULTS: < 0.05). CONCLUSION: -induced liver fibrosis in rats.

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Key concepts: Sirius Red, Hydroxyproline, Hepatic stellate cell, CCL4, Fibrosis, Internal medicine, Hepatic fibrosis, Endocrinology