Potasyum Klorür ve Kalsiyum Sülfatın Daphnia magna (Crustacea: Cladocera) Üzerine Etkileri
Esra Akat, Sena HACIOĞLU, Durdu KORKMAZ
Abstract
Esra Akat, Sena HACIOĞLU, Durdu KORKMAZ
Abstract
Daphnia magna can reproduce rapidly in suitable environment and temperature (15-22 °C). Additionally, D. magna reproduction in laboratory is extremely simple and inexpensive. Moreover, daphnids are frequently used experimental animals in laboratory studies. Therefore, the effects of two different compounds (KCl and CaSO4) on egg production and heart rate in D. magna were examined in this study. Daphnids of treatment groups were exposed to different concentration of KCl and CaSO4 (37.5, 75, 150 mg/L). Ten individuals were used in each group. All experimental samples were made in triplicate. Changes in heart rate were examined for 96 h and the number of heart beats was counted by using a video camera. The number of eggs per individual in each experimental group was recorded for 21 days. As a result of the exposure to CaSO4, an increase in both heart rate and egg reproduction depend on concentration gradients were observed. However, exposure to KCl caused a decrease in the egg production of medium and high-dose groups. Additionally, although an increase in heart rate was examined for two consecutive days after exposure to KCl, a decrease in the heart rate of medium and high-dose groups was observed during the last 48 h.
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Daphnia magna can reproduce rapidly in suitable environment and temperature (15-22 °C). Additionally, D. magna reproduction in laboratory is extremely simple and inexpensive. Moreover, daphnids are frequently used experimental animals in laboratory studies. Therefore, the effects of two different compounds (KCl and CaSO4) on egg production and heart rate in D. magna were examined in this study. Daphnids of treatment groups were exposed to different concentration of KCl and CaSO4 (37.5, 75, 150 mg/L). Ten individuals were used in each group. All experimental samples were made in triplicate. Changes in heart rate were examined for 96 h and the number of heart beats was counted by using a video camera. The number of eggs per individual in each experimental group was recorded for 21 days. As a result of the exposure to CaSO4, an increase in both heart rate and egg reproduction depend on concentration gradients were observed. However, exposure to KCl caused a decrease in the egg production of medium and high-dose groups. Additionally, although an increase in heart rate was examined for two consecutive days after exposure to KCl, a decrease in the heart rate of medium and high-dose groups was observed during the last 48 h.
Key concepts: Cladocera, Daphnia magna, Daphnia, Branchiopoda, Crustacean, Potassium, Calcium, Chemistry