Haematology reference range evaluation for novel research parameters on the complete blood count analyzer sysmex XN-1000

Background: In the field of haematology, there has been a consistent advancement in technology of the complete blood count (CBC) analyzers and hence, there has been an increase in the number of novel parameters that can be measured on these analysers. To put these newer parameters into routine clinical practice, it is essential to evaluate the reference ranges. Aims and Objective: To evaluate the reference intervals (RI) of the parameters which are calculated by Sysmex XN-1000, especially the newer research parameters. Materials and Methods: Blood samples from 150 clinically asymptomatic and apparently healthy individuals were assessed in the study and reference ranges for the same were evaluated on Sysmex XN-1000 CBC analyzer. Appropriate statistical tests were applied for the same. Results: The RIs of CBC parameters were evaluated in 123 adults (65 males and 58 females). Significant gender-dependent differences were found in red blood cells count, packed cell volume, haemoglobin (Hb), platelets and also in reticulocyte Hb and platelet-crit among the newer parameters. The other CBC reference ranges were also assessed from the remaining data. Conclusion: The present study, highlights the significance of different CBC parameters with special emphasis on newer research parameters. Although, the results of the current study are comparable with previous studies; further studies with larger sample sizes are needed for validation purposes in addition to the regional variation pertaining to these parameters.