2022•Pakistan Journal of Analytical & Environmental ChemistryOpen access

Cloud Point Extraction for Spectrophotometric Determination of Acid Blue 29 Dye in Water Samples

Safwan Fraihat, Mohammad Amayreh, Nayyef Al Jaar

Abstract

A simple and fast Cloud Point Extraction (CPE) preconcentration of Acid Blue 29 (AB 29) dye was developed prior to its spectrophotometric determination. The suggested procedure was successfully applied for the determination of AB 29 dye in different water samples with high recoveries. Optimization of experimental conditions such as surfactant type and volume, pH, stirring, and centrifugation conditions were studied. The effect of interfering ions was also studied. The calibration curve was in the range of 0.4-10 µgmL-1 with a limit of detection and limit of quantitation of 0.35 and 1.1 µgmL-1, respectively. Furthermore, the method is free of the interference of common ions present in water.

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Key concepts: Cloud point, Calibration curve, Detection limit, Extraction (chemistry), Chromatography, Chemistry, Pulmonary surfactant, Centrifugation