Study of Detergency. IX

The elution curves in gel filtration using two kinds of bio-bead gel (S-X 2 and S-X 8) were measured for tristearin, fatty acid, cholesterol and its ester which were considered to be the main components of organic soils from the practical application of underwear.When chloroform was applied as the elution solvent, only one peak was observed in the both elution curves in gel filtration of the mixed lipid using S-X 2 and S-X 8. On the other hand, many peaks corresponding to the components contained in lipid mixture were observed in elution curve obtained by the gel filtration using S-X 2 gel and benzene as the elution solvent. The relations between the elution volumes corresponding to peaks in elution curves and molecular weight were discussed.