2010•Methods in molecular biologyRequires access
Production and Purification of Polyclonal Antibodies
Masami Nakazawa, Mari Mukumoto, Kazutaka Miyatake
Open publisher page 7 citations
Abstract
Polyclonal antibodies are derived from multiple B-cell clones that have differentiated into antibody-producing plasma cells in response to an immunogen. Polyclonal antibodies raised against a single molecular species of antigen recognize multiple epitopes on a target molecule resulting in signal amplification in indirect immunoassays, including immuno-electron microscopy. In this chapter, we present a basic procedure to generate polyclonal antibodies in rabbits.