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2014•He'nan nongye kexueRequires access

Synthesis of Complete Antigen and Preparation of Polyclonal Antiserum against Sarafloxacin

Liu Ru-bia

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Abstract

To prepare high sensibility and specificity polyclonal antiserum against Sarafloxacin(SARA),SARA was linked to the carrier proteins BSA and OVA with EDC method to prepare the immunogen SARA-BSA and the coating antigen SARA-OVA.UV scanning spectrum and SDS-PAGE preliminarily proved that SARA was coupled successfully to the carrier protein.The specific polyclonal antiserums with higher titer were obtained by immunizing BALB/c mice with SARA-BSA of 20μg,50μg each mouse respectively,with the titer of 1∶10 000and the inhibition titer of 623.5ng/mL.These results indicated that SARA was successfully linked to the carrier proteins.This study laid the foundation for further gain monoclonal anti-SARA and its immunological assay.

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What this paper is about

To prepare high sensibility and specificity polyclonal antiserum against Sarafloxacin(SARA),SARA was linked to the carrier proteins BSA and OVA with EDC method to prepare the immunogen SARA-BSA and the coating antigen SARA-OVA.UV scanning spectrum and SDS-PAGE preliminarily proved that SARA was coupled successfully to the carrier protein.The specific polyclonal antiserums with higher titer were obtained by immunizing BALB/c mice with SARA-BSA of 20μg,50μg each mouse respectively,with the titer of 1∶10 000and the inhibition titer of 623.5ng/mL.These results indicated that SARA was successfully linked to the carrier proteins.This study laid the foundation for further gain monoclonal anti-SARA and its immunological assay.

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Available abstract

To prepare high sensibility and specificity polyclonal antiserum against Sarafloxacin(SARA),SARA was linked to the carrier proteins BSA and OVA with EDC method to prepare the immunogen SARA-BSA and the coating antigen SARA-OVA.UV scanning spectrum and SDS-PAGE preliminarily proved that SARA was coupled successfully to the carrier protein.The specific polyclonal antiserums with higher titer were obtained by immunizing BALB/c mice with SARA-BSA of 20μg,50μg each mouse respectively,with the titer of 1∶10 000and the inhibition titer of 623.5ng/mL.These results indicated that SARA was successfully linked to the carrier proteins.This study laid the foundation for further gain monoclonal anti-SARA and its immunological assay.

Key concepts: Immunogen, Polyclonal antibodies, Antiserum, Titer, Antigen, Molecular biology, Chemistry, Monoclonal antibody

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Synthesis of Complete Antigen and Preparation of Polyclonal Antiserum against Sarafloxacin — Research Paper | ScholarLens