1983•Agricultural and Biological ChemistryOpen access

Purification and properties of eclosion hormone of the silkworm, Bombyx mori.

Hiromichi Nagasawa, Hajime Fugo, Shigeru Takahashi, Takehiko Kamito, Akira Isogai, Akinori Suzuki

Abstract

Eclosion hormone was purified 5,000-fold from the extracts of male adult heads of the silkworm, Bombyxmori.The fourteen-step purification procedure consisting of solvent extractions, fractional precipitations and chromatographies afforded a partially purified preparation of eclosion hormone, 1.8 fig of which showed activity in a Bombyxpharate adult.The hormone was inactivated by proteolytic enzymes.The molecular weight of eclosion hormone was estimated to be 8,400 ± 1,000 daltons by gel-filtration on Sephadex G-50.

Open-access reader

About this research paper

What this paper is about

Why it matters

OpenAlex reports 21 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: Bombyx mori, Sephadex, Bombycidae, Size-exclusion chromatography, Hormone, Chemistry, Chromatography, Enzyme