Untitled research work

Prostaglandin [1] methel ester (1) and its analogues [2] and [ 3] were easiliy hydrolyzed with pig liver esterase. In this hydrolysis, saturated esters [1] and [ 2] were hydrolyzed about 15 times faster than a, 8-unsaturated ester [ 3]. In the hydrolysis of three other esters [4], [5] and[ 6], the reaction rate decreased in order: saturated ester [4]trans-α, β-unsaturated ester [5], and cis-α, β-unsaturated ester[6]. In comparison with other enzymatic hydrolysis, this method has the advantages of no side reaction and availability of higher substrate concentration.