2009•Acta Phytopathologica SinicaRequires access
Identification and cloning of a NBS resistance gene homology cDNA sequence from wheat
Haiyan Wang, Daqun Liu, Yang WenXiang, Zaifeng Li, Lirong Zhang, Na Zhang
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Abstract
The 5′ and 3′ end of the resistance gene homology fragment S2A2 from TcLr35 were obtained by the rapid amplification cDNA ends(RACE).The gene specific primers were designed based on the spliced sequence,and the full length sequence of S2A2 obtained was 3476bp encoding 866 amino acids.BLASTp analysis,the deduced amino acids of S2A2 protein consisted of a NB-ARC conserved domain and a leucine-rich repeats(LRR) domain,which were identical to the conserved domains of plant resistance genes such as I2C-1,L6,and RPS2.The S2A2 gene appeared not to be induced by Puccinia triticina and was a constitutive gene with low abundance in the wheat leaf tissue by semi-quantitative RT-PCR.In this study,the resistance homo-logy sequence were obtained in TcLr35,which provide the shortcut for cloning of wheat leaf rust resistance gene Lr35.