Cloning and Character of Resistance Homologenes Contained NBS-LRR in Wheat TcLr24

Resistance gene cDNA homology sequence from wheat was isolated using homology-based method.The primers were designed according to the conserved sequence of resistance gene analogs and a 703bp fragment was isolated from TcLr24.A few homologous sequences were captured when processing similarity search in GenBank database.We designed primers based on highly conserved region among the above sequences and the rapid amplification cDNA ends(RACE)was used to obtain the full length sequence of disease resistance homology gene in the TcLr24 near isogenetic lines.Three full length cDNA sequences were obtained.BLASTp analysis showed that the deduced amino acids of protein contained a NBS conserved domain and many leucine-rich repeats(LRR)domains,which were identical to the conserved domains of many plant resistance genes.These sequences appeared not to be induced by Puccinia triticina and were constitutive genes in the wheat leaf tissue by real-time PCR.In this study,we obtained three resistance homology sequences which provide the short cut for researching of wheat resistance gene.