Effects of insitol deficiency on digestive and immune function of juvenile Jian carp (Cyprinus carpio var. Jian).

This study was designed to determine the effects of insitol deficiency on digestive and immune function of juvenile Jian carp(Cyprinus carpio var.Jian).In trial,three hundred and sixty juvenile Jian carps with initial body weight of(10.31±0.04) g were randomly allocated to 2 groups(with 3 replicates and each replicatc had 60 fish) fed with purified diet containing insitol 0 or 450 mg/kg for 80 days.The results showed that,insitol deficiency symptoms of juvenile Jian carp were observed, such as body surface hyperaemia and fin erosion.The insitol deficiency significantly decreased the weight gain and feed intake,and significantly increased the feed conversion ratio(P0.01).The intestine,hepatopancreas weight and protein content were significantly decreased by insitol deficiency(P0.01).The activities of trypase,chymotrypsin,lipase, amylase,alkaline phosphatase,Na~+,K~+ -ATPase,γ-glutamyl transferase and creatinkinase in intestine were all significantly decreased by insitol deficiency(P0.01).The insitol deficiency significantly decreased the kidney,spleen weight before challenge and the survival rate,activity of serum complement C3,C4,IgM-like,acid phosphatase,total iron binding capacity, lectin titer and lysozyme after challenge(P0.01).These results indicated that insitol deficiency resulted in the depres- sion of digestive and immune function of juvenile Jian carp