Study on Pantothenic Acid-Deficiency Signs and Effects of Pantothenic Acid-Deficiency on Intestinal Enzyme Activities and Immune Response of Juvenile Jian Carp

This experiment was conducted with juvenile Jian carp to study pantothenic aicd (PA)-deficiency signs and effects of pantothenic acid -deficiency on intestinal enzyme activities and immune response.Semi -purified diets with three levels (4,25.56 and 65.85 mg PA/kg) were fed to 450 juvenle Jian carp (initial weight 12.95 ± 0.03 g) for 63 days.Juvenile Jian carp fed diet with the dietary PA level of 4 mg/kg exhibited typical signs of PA deficiency such as anorexia,poor growth,exophthalmus,sluggnishness,hemorrhage of body surface and in the fins.Simultaneously,PA significantly decreased weight gain and weight of internal organs.Also Jian carp fed diet with the level of 4 mg PA/kg had the lowest activity of trypsin,lipase,α-amylase,Na+,K+-ATPase (P 0.05) and pathological changes in intestine.After 63-day feeding trial,the challenge experiment with Aeromonas hydrophila (AH) was carried out with juvenile Jian carp,which showed pantothenic acid -deficiency led to high mortality.Contrast to the dietary PA level of 65.85 mg/kg,immune parameter were all decreased by the dietary PA level of 25.56 mg/kg (P 0.05).These results suggested that pantothenic acid is necessary to maintain normal growth,intestinal enzyme activities and immune response of juvenile Jian carp,Cyprinus carpio var.Jian.