Evaluation of ethanolic extract of Citrulluslanatus seeds for analgesic and anti-pyretic activity in animal model

Objective: This currentstudy was design to identify the effect of analgesic and antipyretic activity for ethanolic seed extract of Citrulluslanatus seeds. Methodology: The study was carried out using Sprague-Dawley rats (250-300g) and Albino mice (25-30g). Tramadol and Paracetamol are the standard drugs, was prepared by dissolving in distilled water to make the concentration of 20mg/kg and 150mg/kg for analgesic and antipyretic activity respectively.The effect of ethanolic extract of Citrulluslanatus seeds was investigated for analgesic activity through tail immersion method and for antipyretic activity through yeast induced pyrexia method.Results: The analgesic activity was evaluated using tail immersion method in mice.The ethanolic extract of Citrulluslanatus seeds was shown highly significant (p<0.001) analgesic activity at high dose which is almost comparable to standard drug and at low dose (200 mg/ kg) showed less significant (p<0.05) analgesic activity. In antipyretic activity, the ethanolic extract of Citrulluslanatus seeds was shown highly significant (p<0.001) at high dose and at low dose (200 mg/ kg) respectively.The ethanolic extract of Citrulluslanatus seeds in both low and high dose showed highly significant at 4th hour which is almost comparable to standard drug.Conclusion: The findings of the present study concluded that Citrulluslanatusseeds have potential to treat pain and fever and as a good source, novel natural analgesic and antipyretic agents. The ethanolic extract ofCitrulluslanatusseeds showed highly significant analgesic and antipyretic activity in mice and rats respectively