The Effect of β Cyclodextrin and Oxybenzone-Octyl Dimetyl PABA (3:7% w/w) Addition on the Penetration of Kojic Acid in Vanishing Cream (Based) on Activitu Inhibition of Tyrosinase

This research was aimed to investigat the effects of the addition of β-cyclodextrin (BCD) and a combined of oxybenzone and octyl dimethyl PABA (3:7% w/w) on the penetration of kojic acid based on inhibion activity of tyrosinase. The presence of BCD was expected to make the preparation effective, while the addition of oxybenzone and octyl dimethyl PABA was intended to overcome photosensitive properties of kojic acid. Effectiveness determination of vanishing cream preparation was conducted by testing activity inhibition of tyrosinase that was expressed as percentage inhibition. Tyrosinase was an enzyme that plays an important role in the process of melanin formation in human skin. Inhibition activity of tyrosinase by kojic acid was determined in vitro by observing absorption values of dopachrome (an intermediate product of melanin formation) using spectrophotometer. Based on results of effectiveness tests of kojic­ acid vanishing cream preparation by means of measurement of activity inhibition of tyrosinase, it was found that addition of sunscreen (oxybenzone and octyl dimethyl PABA without BCD) had no significant effect on the activity inhibition of tyrosinase. While, addition of BCD or combined of BCD-sunscreen significantly reduced inhibition of tyrosinase activity in vitro.