Study on the Growth for Selectivity to Negative Ion of Moderately Halophilic Actinomycetes

Cl~-was used as main negative ion long time for isolation and culture of moderately halophilic or halotolerant actinomycetes, but there are other negative ions such as NO_3~-、SO_4~(2-)、CO_3~(2-)and HCO_3~-in salt lake. Media with only Cl~-could not reflect their living natural environments, which was limited to study on moderately halophilic or halotolerant actinomycetes. In this study, the results show that some negative ion, such as NO_3~-and SO_4~(2-), have not much more selectivity to moderately halophilic or halotolerant actinomycetes, Cl~-can be substituted by NO_3~-and SO_4~(2-). CO_3~(2-)and HCO_3~-has little effect on the genus Nocardiopsis,but have much more on other genera such as Prauserella,Streptomonospora and Saccharomonospora, etc. These results will be some instructive to the isolation and cultivation of moderately halophilic or halotolerant actinomycetes.