The preliminary investigation of halophilic and halotolerant Actinomycetes in Yushuihe,Qinghai
Sun Ting
Abstract
Sun Ting
Abstract
Salt ions and pH value of river water,sediment and sandy soil of Yushuihe were measured in this paper,and some preliminary research on halophilic and halotolerant Actinomycetes have been done through design different media,tapy of salt and addition quantity.The results showed that: all the experimental samples are alkaline;the the highest total content of water-soluble salt is sediment which is 158.67 g/kg,then is sandy soil-90.43 g/kg,river water is the lowest one which is 3.1 g/L;as for salt ions,the highest anion content is Cl-,while among the cations,K+ and Na+ are much higher.Therefore,NaCl and KCl are the main existing forms of salt ions in the samples;89 strains of Actinomycetes were obtained and most of them were separated from sediment,and the best isolation medium is modified starch casein medium;8 halophilic Actinomycetes strains were discovered by halophilic and halotolerant Actinomycetes screening,and 3 of them are extreme halophilic Actinomycetes in NaCl while 7 in KCl,and 1 of them is tropic-K Actinomycetes.
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Salt ions and pH value of river water,sediment and sandy soil of Yushuihe were measured in this paper,and some preliminary research on halophilic and halotolerant Actinomycetes have been done through design different media,tapy of salt and addition quantity.The results showed that: all the experimental samples are alkaline;the the highest total content of water-soluble salt is sediment which is 158.67 g/kg,then is sandy soil-90.43 g/kg,river water is the lowest one which is 3.1 g/L;as for salt ions,the highest anion content is Cl-,while among the cations,K+ and Na+ are much higher.Therefore,NaCl and KCl are the main existing forms of salt ions in the samples;89 strains of Actinomycetes were obtained and most of them were separated from sediment,and the best isolation medium is modified starch casein medium;8 halophilic Actinomycetes strains were discovered by halophilic and halotolerant Actinomycetes screening,and 3 of them are extreme halophilic Actinomycetes in NaCl while 7 in KCl,and 1 of them is tropic-K Actinomycetes.
Key concepts: Halophile, Halotolerance, Salt (chemistry), Sediment, Salinity, Chemistry, Starch, Environmental chemistry