Guidelines for Production of Rotifer (Brachionus rotundiformis) in Concentrate Forms to Commercial : Production and Preservation

The objectives of this study were to investigate the production and preservation of marine rotifer ( Brachionus rotundiformis ) in concentrate forms to commercial. The results showed that rotifer cultured by using Nannochloropsis sp. at cell density about 10 5 cell/ml for 3 days was optimal for rotifer production in concentrate forms. The average growth rate and productivity of rotifer were 1.6±0.5 rotifer/day and 117.0±17.4 g/1 ton of water, respectively. The average protein, carbohydrate and lipid contents of rotifer were 32.0±3.8, 12.0±1.9 and 14.9±1.3 % dry weight, respectively. The eicosapentaenoic acid (EPA) and decosahexaenoic acid (DHA) contents of rotifer were 1.4±0.0 and 0.3±0.1 mg/g, respectively. The post-harvest of rotifer in concentrate forms if usage of 15% glycerol, or 3% ascorbic acid as cryoprotectant agents (v/v) for preservation, its optimally stored by biomass-freezing (-18 to -20 oC), and did not affect the cell condition, bacteria and proximate composition in product of rotifer in concentrate forms during stored at 8 weeks. This study indicates that an alternative option is to use production plan to rotifer in concentrate forms production in hatchery for small scale farming or other person in the future. Keywords : rotifer in concentrate forms ; production ; preservation