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Establishment of ISSR-PCR Reaction Conditions in Radix Aconiti Lateralis Preparata

Qun Luo, Huang Chun-ping, Li Jie

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Abstract

ISSR分析可对附子(Radix Aconiti Lateralis Preparata）的遗传多样性进行研究.为获得清晰、重复性高的ISSR扩增结果,对影响ISSR-PCR的多个因素,包括DNA模板浓度、Taq酶用量、引物和Mg2+浓度进行了比较、优化.研究表明,10-60n9模板DNA,1UTaq酶,0.2μmol/L引物,1.5mmol/L MgCl2,4种dNTPs各200μmol/L,10xPCR缓冲液构建的20μL的ISSR反应体系为最适体系.

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What this paper is about

ISSR分析可对附子(Radix Aconiti Lateralis Preparata）的遗传多样性进行研究.为获得清晰、重复性高的ISSR扩增结果,对影响ISSR-PCR的多个因素,包括DNA模板浓度、Taq酶用量、引物和Mg2+浓度进行了比较、优化.研究表明,10-60n9模板DNA,1UTaq酶,0.2μmol/L引物,1.5mmol/L MgCl2,4种dNTPs各200μmol/L,10xPCR缓冲液构建的20μL的ISSR反应体系为最适体系.

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Available abstract

ISSR分析可对附子(Radix Aconiti Lateralis Preparata）的遗传多样性进行研究.为获得清晰、重复性高的ISSR扩增结果,对影响ISSR-PCR的多个因素,包括DNA模板浓度、Taq酶用量、引物和Mg2+浓度进行了比较、优化.研究表明,10-60n9模板DNA,1UTaq酶,0.2μmol/L引物,1.5mmol/L MgCl2,4种dNTPs各200μmol/L,10xPCR缓冲液构建的20μL的ISSR反应体系为最适体系.

Key concepts: Chemistry

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