Binbin Si, Shi Qiaoceng
Abstract
[目的]建立和优化藤三七稳定ISSR-PCR反应体系和扩增程序.[方法]采用5因素4水平的正交试验和单因子梯度优化相结合的方法.[结果]适用于藤三七的25μl ISSR-PCR反应体系中各因素的最佳浓度分别为:10×buffer 2.5μl,dNTPs 0.15 mmoL/L,Mg2+2.0mmol/L,Taq酶1.0U,引物0.5 μmol/L,DNA 100 ng.[结论]对于藤三七ISSR-...
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[目的]建立和优化藤三七稳定ISSR-PCR反应体系和扩增程序.[方法]采用5因素4水平的正交试验和单因子梯度优化相结合的方法.[结果]适用于藤三七的25μl ISSR-PCR反应体系中各因素的最佳浓度分别为:10×buffer 2.5μl,dNTPs 0.15 mmoL/L,Mg2+2.0mmol/L,Taq酶1.0U,引物0.5 μmol/L,DNA 100 ng.[结论]对于藤三七ISSR-...
Key concepts: System optimization, Computer science, Computational biology, Biology, Chemistry, Mathematics, Mathematical optimization