Chemiluminescent self-reporting macromolecular transformation

The present doctoral thesis established advanced optical read-out and characterisation methods for the in-depth analysis of chemical reactions, such as the quantification of reaction events or kinetic analysis, via state-of-the-art chemiluminescence systems. Critically, an optical read-out for the quantification of para-fluoro – thiol reaction events was established employing the chemiluminescence of Schaap’s dioxetane on the one hand, and peroxyoxalate chemiluminescence was employed for the qualitative assessment of single-chain nanoparticle unfolding on the other hand. Both chemiluminescence systems present promising tools for the in-depth characterisation of macromolecular architectures and their transformations.