Cloning and sequence analysis of the RAPD marker linked to anthracnose-resistance gene in Chinese wild Vitis and its application for marker-assisted breeding.

Cloning, sequencing and sequence analysis of the RAPD marker OPS03-1300 linked to anthracnose(Elsinoe ampelina)resistant gene in Chinese wild Vitis were carried out. The result showed that the length of the RAPD marker OPS03-1300 was actually 135 4 bp,so it was renamed as OPS03-1354. Nucleotide sequence data of the RAPD marker had been submitted to the GenBank databases,and the accession number was DQ350885. OPS03-1354 sequence shows identity with 99 EST sequences from Vitis vinifera L.The sequence showed 82% identity with one EST sequence from Cabernet Sauvignon leaves infected with Xylella fastidiosa,80% and 85% identity respectively with two EST sequences from Cabernet Sauvignon berry of different growth stages. Moreover, the sequence showed 67% identity with one EST sequence from Chinese wild Vitis,Vitis pseudoreticulata clone Baihe-35-1 leaves infected with Plasmopara viticola. The sequence can code a hypothetical protein of grape. In addition,Marker-assisted selection of anthracnose-resistance was performed using OPS03-1354 in hybrids from interspecies cross between Chinese wild Vitis and Vitis vinifera,including 339 F1 progenies of combination Guangxi-1×Jingkejing and 207 F2 progenies of combination Baihe-35-1×Carignane.