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2007•Acta Horticulturae SinicaRequires access

Molecular Markers Linked to Drought Resistance Gene in Chinese Wild Vitis Species and Their Genetic Analysis

Liang Yu

Open publisher page 2 citations

Abstract

Random amplified polymorphic DNA(RAPD)and bulked segregant analysis(BSA)were employed to detect molecular marker linked to drought resistance gene in the wild grapes(Vitis L.)native to China. DNA pools related to drought resistance were consisted by using the F_1 progenies of V. yeshanensis×V.riparia. Seven RAPD markers,S226-1100,S271-550, S1165-1500, S264-1300, S195-1000, S1345- 1400 and S513-1700, were obtained and seven primers were screened from 300 random primers. Then the 7 markers were further investigated in a set of 18 genotypes of Chinese wild Vitis species, V. vinifera, V. ber- landieri, and V. rupestris. Additionally, RAPD fragment S226-1100 was cloned and sequenced, then conver- ted into SCAR maker DR-760. Genetic analysis by JoinMap 3.0 showed that RAPD markers S226-1100, S271-550 and S1165-1500 were linked to the drought resistance gene. The linking distance between RAPD marker S226-1100 and the gene locus was 21.8 cM.

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What this paper is about

Random amplified polymorphic DNA(RAPD)and bulked segregant analysis(BSA)were employed to detect molecular marker linked to drought resistance gene in the wild grapes(Vitis L.)native to China. DNA pools related to drought resistance were consisted by using the F_1 progenies of V. yeshanensis×V.riparia. Seven RAPD markers,S226-1100,S271-550, S1165-1500, S264-1300, S195-1000, S1345- 1400 and S513-1700, were obtained and seven primers were screened from 300 random primers. Then the 7 markers were further investigated in a set of 18 genotypes of Chinese wild Vitis species, V. vinifera, V. ber- landieri, and V. rupestris. Additionally, RAPD fragment S226-1100 was cloned and sequenced, then conver- ted into SCAR maker DR-760. Genetic analysis by JoinMap 3.0 showed that RAPD markers S226-1100, S271-550 and S1165-1500 were linked to the drought resistance gene. The linking distance between RAPD marker S226-1100 and the gene locus was 21.8 cM.

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Available abstract

Random amplified polymorphic DNA(RAPD)and bulked segregant analysis(BSA)were employed to detect molecular marker linked to drought resistance gene in the wild grapes(Vitis L.)native to China. DNA pools related to drought resistance were consisted by using the F_1 progenies of V. yeshanensis×V.riparia. Seven RAPD markers,S226-1100,S271-550, S1165-1500, S264-1300, S195-1000, S1345- 1400 and S513-1700, were obtained and seven primers were screened from 300 random primers. Then the 7 markers were further investigated in a set of 18 genotypes of Chinese wild Vitis species, V. vinifera, V. ber- landieri, and V. rupestris. Additionally, RAPD fragment S226-1100 was cloned and sequenced, then conver- ted into SCAR maker DR-760. Genetic analysis by JoinMap 3.0 showed that RAPD markers S226-1100, S271-550 and S1165-1500 were linked to the drought resistance gene. The linking distance between RAPD marker S226-1100 and the gene locus was 21.8 cM.

Key concepts: RAPD, Bulked segregant analysis, Biology, Locus (genetics), Genetics, Genetic marker, Gene, Molecular marker

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