Inhibitory Effects of Transcription Factor E2F-1 siRNA on Invasion and Proliferation of Gastric Cancer Cell Line MGC803

BACKGROUND OBJECTIVE:As an important regulatory factor of cell cycle,transcription factor E2F-1 is closely related to tumorigenesis.This study was to investigate the effects of E2F-1 small interfering RNA(siRNA) on invasion and proliferation of human gastric cancer MGC803 cells.METHODS:E2F-1 siRNA vector containing short hairpin structure was transfected into MGC803 cells.Untransfected and pSilencer4.1-negative-transfected cells were used as controls.The expression of E2F-1 was detected by reverse transcription-polymerase chain reaction(RT-PCR) and Western blot.Cell matrigel invasion assay and cloning assay were used to detect the invasion and proliferation of MGC803 cells after E2F-1 siRNA transfection.RESULTS:At 48 h after E2F-1 siRNA transfection,the mRNA level of E2F-1 was down-regulated by over 90.0% of controls;the protein level of E2F-1 was down-regulated by 79.6% of negative control and by 81.5% of empty control.The number of migrated cells was significantly smaller in E2F-1 siRNA group than in negative and empty control groups(18.0±2.6 vs.48.0±4.6 and 54.0±5.6,P0.05).The number of cell clones was also significantly smaller in E2F-1 siRNA group than in negative and empty control groups(46.0±2.0 vs.122.3±1.5 and 128.7±2.1,P0.05) CONCLUSION:E2F-1 siRNA could down-regulate E2F-1 expression in human gastric cancer MGC803 cells and suppress its invasion and proliferation to some extent.