2008•Zhonghua zhongliu fangzhi zazhiRequires access
Effects of E2F-1 on behaviors of human gastric carcinoma cell line
Li Lei
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Abstract
OBJECTIVE:To explore the effects of E2F-1 on behaviors of human gastric carcinoma cell line MGC-803.METHODS:An eukaryotic expression vector was constructed to transfect a human gastric carcinoma cell line MGC-803.In MGC-803 cells transfected by pCMV-E2F-1-HA,E2F-1 gene was detected by RT-PCR and Western blot techniques.The cell matrigel invasion assay,adhesion assay,migration assay,and cloning assay were used to study the effects of E2F-1 gene.RESULTS:E2F-1 mRNA and protein were detected in MGC-803 cells transfected by pCMV-E2F-1-HA.The invasiveness,migration and proliferation were inhibited significantly in MGC-803 cells transfected by pCMV-E2F-1-HA compared with that in MGC-803 cells transfected by pCMV-HA and untransfected cells,P0.05.The adhesive ability of MGC-803 cells with the matrix was not affected by the transfection,P=0.072.CONCLUSIONS:E2F-1 can affect the behaviors of human gastric carcinoma cell line MGC-803.It suggests that E2F-1 may depress the invasion and metastasis of gastric carcinoma cells.