Isolation and Elucidation of Chemical Constituents of Shandanshaoyao Decoction (II)

Objective:To study the chemical constituents of Shandanshaoyao Decoction.Method:The sample was prepared by boiling water, then precipitated by 75% of ethanol. The ethanol soluble fraction was extracted by chloroform, ethyl acetate and n-butanol, respectively. The extracts were isolated using macroreticular resin, silica gel and polyamide column chromatography, and then the chemical constituents were identified by chemical and spectroscopic methods.Result:Nine compounds were isolated from the n-butanol extract and water soluble fraction, and elucidated as vitexin, quercetin,hyperoside,rutin, paeoniflorin, protocatechaldehyde, (+)-catechin, danshensu and citric acid respectively.Conclusion:The chemical constituents of traditional Chinese medicinal preparations Shandanshaoyao Decoction were reported for the first time. [