Research on Enzyme Extraction Technology of Polyphenols from Lotus Leaf

In order to improve the comprehensive utilization level of lotus resource,lotus leaf was used to extract polyphenols with cellulase,and the effects of cellulase concentration,solid-liquid ratio,pH value,extraction temperature,extraction time and times on the extraction yield of polyphenols were studied,and the optimum extraction parameters were confirmed through the single factor and orthogonal experiments.The results showed that the optimum cellulase concentration was 0.5%,solid-liquid ratio was 1∶30(m/V),pH value was 4.8,extraction temperature was 50 ℃,extraction time was 45 min,extraction times was three.Under this optimum condition,the extraction yield of polyphenols was 2.699%.