The influence of early enteral immunonutrition on immunological function of body and intestine in severely scalded rats

Objective To investigate the influence of early enteral immunonutrition on immunological function of body and intestine in severely scalded rats.Methods Sixty-four healthy adult SD rats were subjected to 30% TBSA full-thickness scald and randomly divided into enteral nutrition group(EN,n=32) and enteral immunonutrition group(EIN,n=32).Another 8 healthy rats were enrolled as normal controls. The changes in CD3~+,CD4~+,CD8~+T lymhocyte subsets,the PCNA expression in intestinal mucosa,the a- mount of plasmocytes in lamina propia of small intestine and the content of s-lgA in small intestine were de- termined on 1,4,7 and 10 post-burn days(PBD).Results(1)CD3~+,CD4~+and the ratio of CD4~+/ CD8~+were remarkably declined,while CD8~+was obviously raised after scald in EN group,and there were statistically significant differences at most of time-points when compared with those in control group(P0.05 or P0.01).CD3~+,CD4~+and the ratio of CD4~+/CD8~+on 7 and 10 PBD in EIN group were obvi- ously higher(increased from 4 PBD on,and it was 1.86±0.25 on 10 PBD)than those in EN group (CD4~+±/CD8~+was 1.26±0.10 on 10 PBD),while CD8~+in EIN group was evidently lower than that in EN group(P0.01).(2)The level of PCNA expression in intestinal mucosa,the amount of plasmocytes in lamina propia of small intestine and the content of s-IgA in small intestine in EIN group were increased sig- nificantly compared with EN group on 4,7,10 PBD(P0.05 or P0.01).Conclusion Early enteral immunonutrition can improve immunological function of body and intestine after severe scald,with better re- suits than enteral nutrition.