The effects of enteral immunonutrition on intestinal mucosa and inflammatory response in scalded rats

Objective:To investigate the effects of enteral immunonutrition on inflammatory reaction and the protective effects for barrier function of intestinal mucosa in rats after thermal injury.Methods:Fifty-four SD rats inflicted by 30% TBSA Ⅲ degree scalds were randomly divided into enteral nutrition (EIN,n=24) group and enteral immunonutrition (EN,n=24) group.Another 6 normal rats were chosen as control group.The rats in EN and EIN groups were fed with isocaloric enteral nutrition in 2 hours after injury.Each 6 rats of three groups were sacrificed at 1st,4th,7th and 10th day before and after injury.The heart blood samples were collected immediately to detect the concentrations of TNF-α,IL-2,IL-6,IL-8 and D-lactic acid in serum.Results:(1)The serum level of TNF-α,IL-6 and IL-8 in both EIN and EN groups were obviously higher than those in control group (P0.01 or P0.05) but the level of IL-2 was decreased (P0.01 or P0.05).The rats in EIN recovered at 7th day (P0.05) after injury and the expression of TNF-α,IL-6 and IL-8 were significantly lower than those in EN (P0.05) at 4th,7th and 10th day.However,the level of IL-2 was obviously increased than the one in EN (P0.05) at same intervals.(2)After injury,the serum level of D-lactic acid increased in control group,which was not reach the same leve l before injury in observation period in EN group and get to the normal level after 4 day in EIN group.Conclusion:Enteral immunonutrition superior to enteral nutrition in the early stage of scalded injury could relieve inflammatory reaction of rats and simultaneously improved the barrier function of intestinal mucosa.