EXPRESSION OF IL-8,IP-10 AND ITS MRNA IN SERUM AND PBMC OF THE PATIENTS WITH PRIMARY HEPATIC CARCINOMA

[Objective] To study the expression of interleukin-8 (IL-8), interferon γ-inducible protein l0 (IP-10) and its mRNA in serum and peripheral blood mononuclear cells (PBMC) of the patients with primary hepatic carcinoma (PHC), and investigate the effect of tumorectomy on the expression. [Methods] A total of 64 cases with primary hepatic carcinoma (PHC) from affiliated hospital of Anhui University of Science and Technology and Huainan Xinhua Hospital form January 2006 to December 2006 were chosen, and 70 healthy people were chosen as control group. Typical PHC patients were selected. The level of IP-10 and IL-8 were detected by ELISA serum, respectively, IL-8 mRNA and IP-10 mRNA were measured by Real-time PCR in PBMC. The level of complementary DNA against GAPDH (lgcDNA/lgGAPDH) was regarded as the extreme level of chemokine. [Results] The levels of IL-8 and IP-10 in serum of PHC patients were (486.2 ± 52.3) pg/ml and (386.7 ± 42.5) pg/ml, respectively. The levels of IL-8 mRNA and IP-10 mRNA in PBMC of PHC patients were 1.135 2 ± 0.139 5 and 0.638 7 ± 0.076 8, respectively. The levels of IL-8 and IP-10 and its mRNA were significantly higher than those in control group (P﹤0.01). The levels of IL-8 and IP-10 and its mRNA increased in 22 cases of patients with PHC after operation because of irritability. The levels of IL-8 and IP-10 in serum and its mRNA in PBMC one week after operation were (869.6 ± 90.7) pg/ml and (439.8 ± 51.5) pg/ml, (1.367 1 ± 0.144 7) and (0.803 2 ± 0.081 7), respectively, which were significantly higher than those of other Postoperative groups (P﹤0.01). [Conclusion] The expression of IP-10, IL-8 and their mRNA in serum of the cases of patients with PHC is significantly higher than those of control group, local tumor excision leads to the rise of expression because of irritability, which decreases gradually after tumor excision