CONSTRUCTION AND IDENTIFICATION OF PCDNA3.1 (+)-MTC28 EUKARYOTIC EXPRESSION VECTOR

[Objective] To construct and identify the pcDNA3.1(+)-MTC28 eukaryotic expression plasmid.[Methods] DNA extracted from M.Tuberculosis was amplified by PCR.Then the PCR products was digested with NheI and EcoRⅠ and cloned into eukaryotic expression plasmid pcDNA3.1(+).[Results] Restriction enzyme digestion and DNA sequencing confirmed that the recombinant eukaryotic expression plasmid PCDNA3.1(+)-MTC28 was constructed correctly.[Conclusion] Successful construction of pcDNA3.1(+)-MTC28 provids the possibility for investigating immunogenicity of the recombinant plasmid and studying on the role of the signal peptide in the protein expression and excretion,and preparation of a new tuberculosis vaccine.