Clinical evaluation of specific IgM treponema pallidum antibody detection

Objective To evaluate the significance of IgM treponema pallidum specific antibody in the diagnosis of syphilis in various periods.Methods By using Western bloting(WB),tolulized red unheated serum test(TRUST),treponema pallidum particle agglutination(TPPA) and treponema pallidum (TP),we examined and analyzed 21 cases of first-stage syphilis, 8 cases of second-stage syphilis, 4 cases of latent syphilis, 3 cases of new born infants suspected with congenital syphilis, 7 cases of syphilis formally treated for 18 months and 5 cases of healthy control groups.Results The positive rates of IgM, TRUST, TPPA and TP in first-stage syphilis cases were 100.0%, 42.9%, 57.1% and 66.7% respectively. The positive rates of IgM, TRUST and TPPA in second-stage syphilis, latent syphilis and congenital syphilis were all 100.0%.The positive rates in syphilis cases received 18 months treatment were 0%(IgM), 28.6%(TRUST) and 100.0%(TPPA). The IgM,TRUST and TPPA in control groups were all negative.Conclusion The specific antibody of IgM treponema pallidum has high sensitivity and specificity,and hence can be applied in the diagnosis of syphilis in various periods.