Evalution of detection of serum IgM Treponema pallidum by several methods in the diagnosis of early congenital syphilis

Objective To evaluate the effects of TPPA-IgM-WB and TP-19(s)-IgM-ELISA in the detection of Treponema pallidum IgM for diagnosis of congenital syphilis.Method Methods of TPPA-IgM-WB,TP-19(s)-IgM-ELISA,FTA-ABS-IgM,TRUST and TPPA were used simultaneously to test sera of 46 neonates born to 45 women with syphilis.Result Positive TP-19(s)-IgM-ELISA(including 21 congenital syphilis) were observed in 24 out of the 46 serum specimens from syphilitic women;23 positive serum IgM were detected by Western blot(21 congenital syphilis).No positive results were observed in the 30 infants from healthy mothers.Conclusion TPPA-IgM-WB and TP-19(s)-IgM-ELISA are sensitive and specific in identification of symptomatic treponema pallidum infected infants.The diagnostic value of TPPA-IgM-WB and TP-19(s)-IgM-ELISA in detection of Treponema pallidum serum IgM is superior to any one of the conventional methods.