Interaction between p53 and HSP70 in human hepatocellular carcinoma tissues

AIM: To investigate the interaction between p53 and heat shock protein70 (HSP70) in human hepatocellular carcinoma tissues. METHODS: HSP70 and p53 double positive samples were screened by imunohistochemical staining from twelve human hepatocellular carcinoma tissues, and the tissue homogenate was prepared. Coimmunopitation was performed by using either anti HSP70 mAb or anti p53 mAb. The immunoprecipitate was analysed by SDS PAGE and Western blot. RESULTS: Immunohistochemical staining detection showed that three samples out of twelve cases were double positive for both HSP70 and p53. p53 protein existed in the immunoprecipitate of anti HSP70 mAb, while there was HSP70 protein in the immunoprecipitate of anti p53 mAb. CONCLUSION: HSP70 and p53 exist as a complex in human hepatocellular carcinoma tissues, which will provide a new route for studying the pathogenesis and immunotherapy of human hepatocellular carcinoma.