An experiment study on wound healing with exogenous hyaluronic acid

Objective To evaluate the effects and possible mechanisms of hyaluronic acid (HA) to inhibit scar formation as well as the dose dependent relationship.Methods Forty five health adult SD rats in a dorsal trauma model were randomly divided into the 2% HA treated group( group A),1% HA treated group(group B),and the PBS treated group as a control group(group C).The process of healing was observed,the remained wound areas were measured,and a mean wound healing time was calculated.Furthermore, the dynamic analysis was performed on local tissue protein,HPr and PCⅢ contents.Results ①The remained wound areas of the control group were much smaller than that of group A and B ,and the mean value of wound healing time was 15 2±2 0,14 2±1 6 and 13 4±1 4 days respectively.②Histologically,when compared qualitatively with PBS controls,HA treated wounds exhibited a less inflammatory response and angiogenesis. The fibroblast proliferated significantly,and the collagen fibril was slender and arrayed in good order,③The contents of tissue HPr were statistically lower in HA treated groups,while the contents of PCⅢ were statistically higher compared to the control group.Conclusions The study indicated that ①The exogenous hyaluronan acid may effectively inhibit collagen synthesis and increase tissue PCⅢ content.As a result,the scar formation was decreased.②Hyaluronal efficacy presented a dose dependent manner.\;