Biological effect of hyaluronic acid on wound healing

Objective To investigate the effect of hyaluronic acid(HA) and hyaluronidase(HAse) on wound healing.?Methods Wound healing models were established on ears of 18 adult rabbits,which were randomly divided into the HA treated group(group A),the HAse treated group(group B),the PBS treated group(group C).?The process of wound healing was observed morphologically and histologically.?The variation of the contents of hydroxyproline(HPr) at the stage of wound healing and after healing was measured.?Results (1)?The mean values of wound healing time of group A,B and C were (11.7±0.6),(9.8±0.9),(10.8±1.0) days respectively.?(2)?Compared with PBS controls and HAse treated group,the collagen fibril was slender and arrayed in good order in HA treated wound at early stage of wound healing,but there were no difference among them at the stage of scar formation.?(3)?The contents of HPr were statistically lower in HA treated group than in controls at the stage of wound healing,but no difference at the stage of scar formation.?The HPr contents in HAse treated group were higher than the other groups all the way.?Conclusion HA could suppress the synthesis of collagen,improve the collagen arrangement,delay wound healing,and decrease scar tissue formation.