2000•PROGRESS IN BIOCHEMISTRY AND BIOPHYSICSRequires access
DNA fingerprinting in chicken with probes derived from randomly amplified polymorphic DNAs-PCR products
Shaoqing Yang, Anming Meng
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Abstract
Four chickens, each from a different strain, were analyzed using randomly amplified polymorphic DNAs (RAPD) technique with 12 primers. 38 of 99 (38%) detected fragments showed polymorphic. Four individual-specific fragments were recovered and probed with labeled whole genome of chicken. Positive signal was detected in three of the fragments, suggesting that hypervariable fragments generated by RAPD contain repetitive sequences. Highly variable DNA fingerprints were generated when such repetitive sequence containing fragments were used repetitive sequence containing fragments were used as probe to hybridize to HaeIII-digests of random chickens^DNA.