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DNA Fingerprinting in Chicken with Probes Derived from Randomly Amplified Polymorphic DNAs PCR Products.

Yang Washington Shao, Meng Nan An

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Abstract

Four chickens, each from a different strain, were analyzed using randomly amplified polymorphic DNAs(RAPD) technique with 12 primers. 38 of 99(38%) detected fragments showed polymorphic. Four individual specific fragments were recovered and probed with labeled whole genome of chicken. Positive signal was detected in three of the fragments, suggesting that hypervariable fragments generated by RAPD contain repetitive sequences. Highly variable DNA fingerprints were generated when such repetitive sequence containing fragments were used as probe to hybridize to HaeⅢ digests of random chickensDNA. Therefore, RAPD products that are highly variable among individuals can be used as DNA fingerprinting probes.

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What this paper is about

Four chickens, each from a different strain, were analyzed using randomly amplified polymorphic DNAs(RAPD) technique with 12 primers. 38 of 99(38%) detected fragments showed polymorphic. Four individual specific fragments were recovered and probed with labeled whole genome of chicken. Positive signal was detected in three of the fragments, suggesting that hypervariable fragments generated by RAPD contain repetitive sequences. Highly variable DNA fingerprints were generated when such repetitive sequence containing fragments were used as probe to hybridize to HaeⅢ digests of random chickensDNA. Therefore, RAPD products that are highly variable among individuals can be used as DNA fingerprinting probes.

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Available abstract

Four chickens, each from a different strain, were analyzed using randomly amplified polymorphic DNAs(RAPD) technique with 12 primers. 38 of 99(38%) detected fragments showed polymorphic. Four individual specific fragments were recovered and probed with labeled whole genome of chicken. Positive signal was detected in three of the fragments, suggesting that hypervariable fragments generated by RAPD contain repetitive sequences. Highly variable DNA fingerprints were generated when such repetitive sequence containing fragments were used as probe to hybridize to HaeⅢ digests of random chickensDNA. Therefore, RAPD products that are highly variable among individuals can be used as DNA fingerprinting probes.

Key concepts: RAPD, DNA profiling, Biology, DNA, Hypervariable region, Molecular biology, Genetics, Gene

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DNA Fingerprinting in Chicken with Probes Derived from Randomly Amplified Polymorphic DNAs PCR Products. — Research Paper | ScholarLens