2015Nanfang nongye xuebaoRequires access

GC-MS analysis and antioxidant activity of volatile oil from leaves of Canarium pimela Leenh. in Rongxian county

LI Zhi-fe

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Abstract

【Objective】The chemical constituents and antioxidant activity of the volatile oil from leaves of Canarium pimela Leenh. in Rong County were studied to provide references for its medicinal development. 【Method 】The volatile oil was extracted by steam distillation, and then the composition of oil was analyzed by GC-MS. Chromatographic peak area normalization method was used to calculate the relative mass fraction of each ingredient. The DPPH· method was used to study its antioxidant activity. 【 Result 】 55 compounds were isolated and 53 compounds were identified in the leaves that composed about 98.130% of the total volatile oils, in which the ratio of monoterpenoids and sesquiterpenes was relatively higher, 20 and 18 types, respectively. The principal chemical constituents of the volatile oil were eremophila-1(10), 11-diene(11.652%),α-selinene(10.291%), γ-muurolene(9.719%), α-pinene(9.208%) and1,2,3,4,4a,7-hexahydro-1,6-dimethyl-4-(1-methylethyl)-Naphthalene(7.523%). The scavenging ratio for the DPPH· radicals reached 63 % at the concentration of 1. 2 g / L. 【 Conclusion 】 There are many chemical constituents in the volatile oil and the main compounds are monoterpenoids. The volatile oil shows certain antioxidant activity and can be further studied as a natural spice and antioxidant.

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【Objective】The chemical constituents and antioxidant activity of the volatile oil from leaves of Canarium pimela Leenh. in Rong County were studied to provide references for its medicinal development. 【Method 】The volatile oil was extracted by steam distillation, and then the composition of oil was analyzed by GC-MS. Chromatographic peak area normalization method was used to calculate the relative mass fraction of each ingredient. The DPPH· method was used to study its antioxidant activity. 【 Result 】 55 compounds were isolated and 53 compounds were identified in the leaves that composed about 98.130% of the total volatile oils, in which the ratio of monoterpenoids and sesquiterpenes was relatively higher, 20 and 18 types, respectively. The principal chemical constituents of the volatile oil were eremophila-1(10), 11-diene(11.652%),α-selinene(10.291%), γ-muurolene(9.719%), α-pinene(9.208%) and1,2,3,4,4a,7-hexahydro-1,6-dimethyl-4-(1-methylethyl)-Naphthalene(7.523%). The scavenging ratio for the DPPH· radicals reached 63 % at the concentration of 1. 2 g / L. 【 Conclusion 】 There are many chemical constituents in the volatile oil and the main compounds are monoterpenoids. The volatile oil shows certain antioxidant activity and can be further studied as a natural spice and antioxidant.

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Available abstract

【Objective】The chemical constituents and antioxidant activity of the volatile oil from leaves of Canarium pimela Leenh. in Rong County were studied to provide references for its medicinal development. 【Method 】The volatile oil was extracted by steam distillation, and then the composition of oil was analyzed by GC-MS. Chromatographic peak area normalization method was used to calculate the relative mass fraction of each ingredient. The DPPH· method was used to study its antioxidant activity. 【 Result 】 55 compounds were isolated and 53 compounds were identified in the leaves that composed about 98.130% of the total volatile oils, in which the ratio of monoterpenoids and sesquiterpenes was relatively higher, 20 and 18 types, respectively. The principal chemical constituents of the volatile oil were eremophila-1(10), 11-diene(11.652%),α-selinene(10.291%), γ-muurolene(9.719%), α-pinene(9.208%) and1,2,3,4,4a,7-hexahydro-1,6-dimethyl-4-(1-methylethyl)-Naphthalene(7.523%). The scavenging ratio for the DPPH· radicals reached 63 % at the concentration of 1. 2 g / L. 【 Conclusion 】 There are many chemical constituents in the volatile oil and the main compounds are monoterpenoids. The volatile oil shows certain antioxidant activity and can be further studied as a natural spice and antioxidant.

Key concepts: Chemistry, DPPH, Steam distillation, Antioxidant, Gas chromatography–mass spectrometry, Essential oil, Chemical composition, Food science

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