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Effects of Dimethyloxalyl Glycine on Hypoxic-ischemic Brain Damage in Newborn Rats

MU De-zh

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Abstract

Objective To investigate the effects of dimethyloxalyl glycine on hypoxic-ischemic brain damage in newborn rats.Methods Forty eight postnatal day 10 SD rats were divided into 3 groups,including sham surgery group,hypoxic-ischemic group and DMOG treated group.The brain tissues were collected at 4,8,24 and 72 hours after the hypoxic-ischemic treatment.The expressions of hypoxia inducible factor-1α(HIF-1α) protein and anti-apoptoticprotein cleaved caspase 3(CC3) were detected by immunohistochemistry.The apoptotic cells were detected by TUNEL staining.Results The expression level of HIF-1α was significantly higher in DMOG treated group than in hypoxic-ischemic group.While the expression level of CC3 was lower and the number of tunel positive cells was fewer in DMOG treated group than that in hypoxic-ischemic group.Conclusion Dimethyloxalyl glycine may play a neuro-protective role in hypoxic-ischemic brain damage in newborn rats by stabilizing HIF-1α.

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What this paper is about

Objective To investigate the effects of dimethyloxalyl glycine on hypoxic-ischemic brain damage in newborn rats.Methods Forty eight postnatal day 10 SD rats were divided into 3 groups,including sham surgery group,hypoxic-ischemic group and DMOG treated group.The brain tissues were collected at 4,8,24 and 72 hours after the hypoxic-ischemic treatment.The expressions of hypoxia inducible factor-1α(HIF-1α) protein and anti-apoptoticprotein cleaved caspase 3(CC3) were detected by immunohistochemistry.The apoptotic cells were detected by TUNEL staining.Results The expression level of HIF-1α was significantly higher in DMOG treated group than in hypoxic-ischemic group.While the expression level of CC3 was lower and the number of tunel positive cells was fewer in DMOG treated group than that in hypoxic-ischemic group.Conclusion Dimethyloxalyl glycine may play a neuro-protective role in hypoxic-ischemic brain damage in newborn rats by stabilizing HIF-1α.

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Available abstract

Objective To investigate the effects of dimethyloxalyl glycine on hypoxic-ischemic brain damage in newborn rats.Methods Forty eight postnatal day 10 SD rats were divided into 3 groups,including sham surgery group,hypoxic-ischemic group and DMOG treated group.The brain tissues were collected at 4,8,24 and 72 hours after the hypoxic-ischemic treatment.The expressions of hypoxia inducible factor-1α(HIF-1α) protein and anti-apoptoticprotein cleaved caspase 3(CC3) were detected by immunohistochemistry.The apoptotic cells were detected by TUNEL staining.Results The expression level of HIF-1α was significantly higher in DMOG treated group than in hypoxic-ischemic group.While the expression level of CC3 was lower and the number of tunel positive cells was fewer in DMOG treated group than that in hypoxic-ischemic group.Conclusion Dimethyloxalyl glycine may play a neuro-protective role in hypoxic-ischemic brain damage in newborn rats by stabilizing HIF-1α.

Key concepts: TUNEL assay, Hypoxia (environmental), Brain damage, Immunohistochemistry, Apoptosis, Medicine, Glycine, Anesthesia

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Effects of Dimethyloxalyl Glycine on Hypoxic-ischemic Brain Damage in Newborn Rats — Research Paper | ScholarLens