2012•Acta Scientiarum Naturalium Universitatis SunyatseniRequires access
Preparation of Active Peptides from Corn and Soybean by Bi-enzyme Hydrolysis
Mu, Mingfeng, Gao, Yun, Minglun Gong, Guan, Meng Meng, Jian-biao, Zhang, Peng ......................................................
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Abstract
[Objective] The aim was to study the optimal test condition for preparation of active peptides from corn and soybean by bi-enzyme hydrolysis.[Method] Taking soybean peptide and corn peptide as materials,different concentrations of soy-corn protein isolate solution were prepared.Through pretreatment,and based on compound protease,flavor protease,neutral protease,papaya protease,pineapple hydrolysis,and by measuring the degree of hydrolysis activity and enzyme activity,tested enzyme was determined.Mixing two of those enzymes in certain proportion and by orthogonal test,the optimal condition of enzyme hydrolysis of a double was determined.[Result] The optimal hydrolysis condition was 2% of substrate concentration,1:1of hydrolyze compound protease and flavor protease,and 8 h of hydrolysis time,75.14% of DH.[Conclusion] The study provided a basis for the development and application of active peptides from corn and soybean by bi-enzyme hydrolysis.