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Double Enzyme Hydrolysis for Preparation of Corn Peptides

Guang Chen

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Abstract

Double enzyme hydrolysis was used to prepare peptides from corn gluten meal.In order to maximize degree of hydrolysis(DH),crucial hydrolysis parameters were optimized.Results showed that a maximum DH of 80.97% was obtained by optimized sequential hydrolysis for 1.5 h using protease alkaline at 55 ℃ and pH 8.5 with 5 g/100mL substrate concentration and 4% enzyme dose followed by 1.5 h using papain at pH 7.5 with 3.5% enzyme dose,which was significantly higher that obtained by single enzyme hydrolysis.

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What this paper is about

Double enzyme hydrolysis was used to prepare peptides from corn gluten meal.In order to maximize degree of hydrolysis(DH),crucial hydrolysis parameters were optimized.Results showed that a maximum DH of 80.97% was obtained by optimized sequential hydrolysis for 1.5 h using protease alkaline at 55 ℃ and pH 8.5 with 5 g/100mL substrate concentration and 4% enzyme dose followed by 1.5 h using papain at pH 7.5 with 3.5% enzyme dose,which was significantly higher that obtained by single enzyme hydrolysis.

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Available abstract

Double enzyme hydrolysis was used to prepare peptides from corn gluten meal.In order to maximize degree of hydrolysis(DH),crucial hydrolysis parameters were optimized.Results showed that a maximum DH of 80.97% was obtained by optimized sequential hydrolysis for 1.5 h using protease alkaline at 55 ℃ and pH 8.5 with 5 g/100mL substrate concentration and 4% enzyme dose followed by 1.5 h using papain at pH 7.5 with 3.5% enzyme dose,which was significantly higher that obtained by single enzyme hydrolysis.

Key concepts: Papain, Hydrolysis, Chemistry, Chromatography, Enzyme, Substrate (aquarium), Enzymatic hydrolysis, Protease

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