Study on the callus induction and plantlet regeneration from the bulbil of Dioscorea opposita

This paper deals with studies on \%in vitro\% culture of bulbil of Dioscorea opposita ,including callus induction and differentiation,plantlet root development and transplantation.Results showed:(1) Calli were obtained from bulbil on media containing different kinds of auxin and cytokinin,also the plantlets could be developed at the same time.It was advantageous to callus induction on MS basal medium supplemented with BA 2 mg/L and NAA 2 mg/L.The frequency of callus induction was 100%;(2) The combination of BA 1 mg/L and NAA 1 mg/L was the best to callus differentiation of which ratio was 63.6%.Most of calli formed multiple buds;(3) 91.2% of the regenerated plantlets treated with KT 2 mg/L+NAA 0.02 mg/L+PP333 0.1 mg/L could develop their roots,grew robustly and robusted green leaves;after the regenerated plantlets were transplanted to soil,they grew robustly and robusted green leaves,moreover could adapt to enviroment.