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Rapid Amplification of 3'cDNA End of BmKIT3 by Two-step PCR.

Zhu Shun

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Abstract

A modified method of 3′ RACE (rapid amplification of 5′ cDNA ends) can rapidly amplify 3′ end of a cDNA only by using a specific primer and Oligo dT.Contrast with the typical 3′ RACE,two step PCR not only have the advantages of less cost and shorter experimental time ,but can also improve specificity of PCR. This method especially fits for rapid cloning and characterization of the genes encoding the peptides with biological activity.

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What this paper is about

A modified method of 3′ RACE (rapid amplification of 5′ cDNA ends) can rapidly amplify 3′ end of a cDNA only by using a specific primer and Oligo dT.Contrast with the typical 3′ RACE,two step PCR not only have the advantages of less cost and shorter experimental time ,but can also improve specificity of PCR. This method especially fits for rapid cloning and characterization of the genes encoding the peptides with biological activity.

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Available abstract

A modified method of 3′ RACE (rapid amplification of 5′ cDNA ends) can rapidly amplify 3′ end of a cDNA only by using a specific primer and Oligo dT.Contrast with the typical 3′ RACE,two step PCR not only have the advantages of less cost and shorter experimental time ,but can also improve specificity of PCR. This method especially fits for rapid cloning and characterization of the genes encoding the peptides with biological activity.

Key concepts: Complementary DNA, Rapid amplification of cDNA ends, Primer (cosmetics), Molecular biology, Cloning (programming), Biology, Primer dimer, Polymerase chain reaction

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Rapid Amplification of 3'cDNA End of BmKIT3 by Two-step PCR. — Research Paper | ScholarLens