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2006•Hebei Journal of Forestry and Orchard ResearchRequires access

Research progress of rapid amplification of cDNA ends

Min Hao

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Abstract

Rapid amplification of cDNA ends is a method obtaining 3′ ends and 5′ ends of known cDNA fragments based on PCR reaction,and one of the primary means cloning cDNA fragments.Beginning with the principle this article analyses the three critical steps(transcription,TdT tailing,PCR amplification) of RACE technique in detail,and expatiates the latest RACE techniques(Marathon-PCR,SMART-RACE,In silico cloning).

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What this paper is about

Rapid amplification of cDNA ends is a method obtaining 3′ ends and 5′ ends of known cDNA fragments based on PCR reaction,and one of the primary means cloning cDNA fragments.Beginning with the principle this article analyses the three critical steps(transcription,TdT tailing,PCR amplification) of RACE technique in detail,and expatiates the latest RACE techniques(Marathon-PCR,SMART-RACE,In silico cloning).

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Available abstract

Rapid amplification of cDNA ends is a method obtaining 3′ ends and 5′ ends of known cDNA fragments based on PCR reaction,and one of the primary means cloning cDNA fragments.Beginning with the principle this article analyses the three critical steps(transcription,TdT tailing,PCR amplification) of RACE technique in detail,and expatiates the latest RACE techniques(Marathon-PCR,SMART-RACE,In silico cloning).

Key concepts: Rapid amplification of cDNA ends, Complementary DNA, Cloning (programming), Biology, Computational biology, Molecular biology, Polymerase chain reaction, Genetics

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