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Determination of Auramine O and Acid Orange II in Yellow Croaker by LC-MS/MS

Wang Duo-jia

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Abstract

A high performance liquid chromatography tandem quadrupole mass spectrometry method was developed for rapid determination of Auramine O and Acid orangeⅡ in yellow croaker. The sample was extracted by acetonitrile, the fat was removed through freezing at-20 ℃, then analyzed by LC-MS/MS. The results showed good linearity in the ranges of 0.5 μg/kg-10 μg/kg,5 μg/kg-100 μg/kg for Auramine O and Acid orange Ⅱ,respectively. The recoveries were between 70 % and 110 %, and the value of the RSD(n=5) was lower than 15 %for the both. The limit of quantitative(LOQ) for Auramine O and Acid orangeⅡ was 0.5 μg/kg and 5 μg/kg,separately. The method was suitable to detect Auramine O and Acid orange Ⅱas two banned industrial dyes in yellow croaker.

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What this paper is about

A high performance liquid chromatography tandem quadrupole mass spectrometry method was developed for rapid determination of Auramine O and Acid orangeⅡ in yellow croaker. The sample was extracted by acetonitrile, the fat was removed through freezing at-20 ℃, then analyzed by LC-MS/MS. The results showed good linearity in the ranges of 0.5 μg/kg-10 μg/kg,5 μg/kg-100 μg/kg for Auramine O and Acid orange Ⅱ,respectively. The recoveries were between 70 % and 110 %, and the value of the RSD(n=5) was lower than 15 %for the both. The limit of quantitative(LOQ) for Auramine O and Acid orangeⅡ was 0.5 μg/kg and 5 μg/kg,separately. The method was suitable to detect Auramine O and Acid orange Ⅱas two banned industrial dyes in yellow croaker.

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Available abstract

A high performance liquid chromatography tandem quadrupole mass spectrometry method was developed for rapid determination of Auramine O and Acid orangeⅡ in yellow croaker. The sample was extracted by acetonitrile, the fat was removed through freezing at-20 ℃, then analyzed by LC-MS/MS. The results showed good linearity in the ranges of 0.5 μg/kg-10 μg/kg,5 μg/kg-100 μg/kg for Auramine O and Acid orange Ⅱ,respectively. The recoveries were between 70 % and 110 %, and the value of the RSD(n=5) was lower than 15 %for the both. The limit of quantitative(LOQ) for Auramine O and Acid orangeⅡ was 0.5 μg/kg and 5 μg/kg,separately. The method was suitable to detect Auramine O and Acid orange Ⅱas two banned industrial dyes in yellow croaker.

Key concepts: Orange (colour), Chemistry, Chromatography, Detection limit, Mass spectrometry, Liquid chromatography–mass spectrometry, Tandem mass spectrometry, Food science

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