Fast Determination of Three Acid Orange Dyes:Acid Orange II,Acid Orange III and Acid Orange AGT in Meat Products by Ion-pair Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

A method based on ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) was developed for the simultaneous determination of three industrial acid dyes,namely acid orange Ⅱ,acid orange Ⅲ and acid orange AGT,in meat products.The samples were extracted with acetonitrile(containing 5% ammonia),cleaned up and enriched with WAX solid phase extraction(SPE) cartridge,and then analyzed by UPLC-MS/MS under the multiple reaction monitoring(MRM) mode.All the analytes were successfully detected in negative ion mode by adding an ion-pair reagent of dihexylammonium acetate(DHAA) to the mobile phase in 6 min.The calibration curves were linear between 5.0 μg/L and 300 μg/L,and the correlation coefficients(r) were more than 0.999.The limits of detection(LOD,S/N=3) and the limits of quantitation(LOQ,S/N=10) were in the range of 20.0-50.0 μg/kg and 70.0-170.0 μg/kg,respectively.The spiked recoveries were in the range of 83%-89%,and relative standard deviations(RSD,n=3) were no more than 7.5%.The method was simple,effective and sensitive,and was suitable in the determination and confirmation of three industrial acid orange dyes in the meat product samples.