The effect of drought stress on protective enzymes system in roots, stems and leaves of Elaeagnus angustifolia L. seedlings.
Jingkuan Sun, Jiangbao Xia, Tian JiaYi, Tian Li
Abstract
Jingkuan Sun, Jiangbao Xia, Tian JiaYi, Tian Li
Abstract
Under different concentrations of PEG-6000 solution simulated drought stress conditions,protective enzymes and malondialdehyde(MDA) of Elaeagnus angustifolia L.in leaves,stems and roots were studied,the results showed:(1)Along with the increasing degree of drought stress,activity of superoxide dismutase(SOD),catalase(CAT) in leaves always increased,activity of perocidase(POD) first decreased,then increased;(2)Under light drought stress,activity of SOD in stems was higher than that of the control,activity of SOD under moderate stress and severe stress in stems was lower than that of the control,activity of POD and CAT always increased,which showed that activity of SOD decreased under moderate and severe stress,but the capacity of POD and CAT to remove into H2O2 was increasing;(3)Along with the increasing degree of drought stress,activity of SOD in roots was higher than that of the control,activity of SOD in roots was not different significantly with that of the control under moderate stress and severe stress,activity of POD was significantly higher than that of the control under different drought stress,but the difference between drought stress was not significant,activity of CAT first decreased,then increased,which was still higher than that of the control;(4)Under light drought stress,membrane lipid peroxidation of leaves,stems,roots of Elaeagnus angustifolia was lower,which was not significantly different from that of the control,and membrane lipid peroxidation of stems,roots increased(stemroot)under moderate drought stress,membrane lipid peroxidation of leaves,stems,roots was significantly increased(leaf stemroot)under severe drought stress.
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Under different concentrations of PEG-6000 solution simulated drought stress conditions,protective enzymes and malondialdehyde(MDA) of Elaeagnus angustifolia L.in leaves,stems and roots were studied,the results showed:(1)Along with the increasing degree of drought stress,activity of superoxide dismutase(SOD),catalase(CAT) in leaves always increased,activity of perocidase(POD) first decreased,then increased;(2)Under light drought stress,activity of SOD in stems was higher than that of the control,activity of SOD under moderate stress and severe stress in stems was lower than that of the control,activity of POD and CAT always increased,which showed that activity of SOD decreased under moderate and severe stress,but the capacity of POD and CAT to remove into H2O2 was increasing;(3)Along with the increasing degree of drought stress,activity of SOD in roots was higher than that of the control,activity of SOD in roots was not different significantly with that of the control under moderate stress and severe stress,activity of POD was significantly higher than that of the control under different drought stress,but the difference between drought stress was not significant,activity of CAT first decreased,then increased,which was still higher than that of the control;(4)Under light drought stress,membrane lipid peroxidation of leaves,stems,roots of Elaeagnus angustifolia was lower,which was not significantly different from that of the control,and membrane lipid peroxidation of stems,roots increased(stemroot)under moderate drought stress,membrane lipid peroxidation of leaves,stems,roots was significantly increased(leaf stemroot)under severe drought stress.
Key concepts: Point of delivery, Catalase, Malondialdehyde, Superoxide dismutase, Lipid peroxidation, Horticulture, Drought stress, Enzyme assay